Composite

Part:BBa_K1316011:Design

Designed by: Joan Cortada Garcia   Group: iGEM14_TU_Delft-Leiden   (2014-09-13)


pFAB640 regulating the ccmAH cluster


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 730
    Illegal BamHI site found at 3519
    Illegal BamHI site found at 3747
    Illegal BamHI site found at 6091
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 384
    Illegal AgeI site found at 1211
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4798


Design Notes

An NdeI restriction site was placed between the promoter and the ccm cluster to be able to easily exchange the promoter


Source

Caroline M. Ajo-Franklin laboratory (Goldbeck et al. ACS Synth. Biol. 2013, 2, 150−159) and Edinburgh 2012 iGEM team

References